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Telephone: 800-DNA-PREP (800-362-7737). Contact Technical Service Contact Customer Care. Read independent reviews on Rotor-Gene™ 6000 - the future of real-time genetic analysis from QIAGEN on SelectScience. Software: Hailed as the best.QIAGEN's real-time PCR cycler, the Rotor-Gene Q, combines multiple optimized design features to provide the outstanding performance and reliable results that your research demands. Together with optimized QIAGEN kits for real-time PCR, the Rotor-Gene Q enables streamlined analysis for a wide range of applications.Is the new operating and analysis software for life science qPCR applications, with a plug-in concept that lets you add new functionality without affecting established workflows.
Explore the to learn more about the Rotor-Gene Q. A human A/T SNP in the AHRR7 gene was analyzed using genomic DNA from wild-type (blue), homozygous mutant (green), and heterozygous (red) samples.
Experiments were performed using the Type-it HRM PCR Kit and a Rotor-Gene Q cycler with an HRM channel. Data analysis was performed with the unsupervised mode of Rotor-Gene ScreenClust HRM Software. A/T polymorphisms (class IV SNPs) are most difficult to discriminate due to minute differences between homozygote alleles (in this example, less than 0.1°C).A HRM raw dataB cluster plot. All pseudo-unknowns were correctly clustered according to genotype. Twofold dilutions of human genomic DNA from 30 ng (10,000 copies) to 0.06 ng (20 copies) were used as a template in real-time PCR. Five replicate reactions were run for each dilution using a self-designed TaqMan® assay for IL1R2 and the Rotor-Gene Probe PCR kit on the Rotor-Gene Q.
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The average difference in the C T values between all dilutions was 1.07 cycles. Human genomic DNA was used as template in 72 replicate real-time PCRs using a self-designed TaqMan® assay for BCL2 on the Rotor-Gene Q without ROX normalization.The average C T value was 24.94 with a standard deviation of only 0.05, equivalent to a CV of 0.2%. The Rotor-Gene Q is the only real-time cycler currently capable of deciphering the most difficult class IV SNPs by HRM.Harness the power of HRM using dedicated QIAGEN HRM Kits for applications such as genotyping (see figure ' for data from the Type-it HRM PCR Kit), quantitative methylation analysis (see figure ' for data from the EpiTect HRM PCR Kit), gene scanning, and sequence matching.
Rotor Gene 6000
The Type-it HRM PCR Kit reliably and accurately detects gene mutations and SNPs. The EpiTect HRM PCR Kit enables fast screening and accurate detection of changes in CpG methylation status of bisulfite converted DNA. Unique rotary design for outstanding performance The unique centrifugal rotary design of the Rotor-Gene Q makes it the most precise and versatile real-time PCR cycler currently available (see figure ').Each tube spins in a chamber of moving air, keeping all samples at precisely the same temperature during rapid thermal cycling. Detection is similarly uniform. When each tube aligns with the detection optics, the sample is illuminated and the fluorescent signal is rapidly collected from a single, short optical pathway.
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This thermal and optical uniformity results in sensitive, precise, and fast real-time PCR analysis (see figure '). It also eliminates sample-to-sample variations and edge effects.